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ATCCCCL-139 TCMK-1 ATCC細(xì)胞購(gòu)買(mǎi)
| 基本信息 | |
| 資源編號(hào) | ATCCCCL-139 |
| 資源名稱(chēng) | TCMK-1 |
| 種屬 | Mus musculus, mouse kidney |
| 類(lèi)型 | SV40 transformed |
| 形態(tài) | epithelial |
| 提供形式 | frozen |
| 致病類(lèi)型 | normal |
| 安全等級(jí) | 2 |
| 培養(yǎng)方法 | |
| 培養(yǎng)基 | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| 傳代方法 | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| 生長(zhǎng)條件 | Temperature: 37°C |
| 生長(zhǎng)特性 | adherent |
| 存儲(chǔ)條件 | liquid nitrogen vapor phase;Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| 詳細(xì)說(shuō)明 | |
| 描述 | About 2% of the cells are positive for SV40 viral antigen, but virus is not recoverable. Tested and found negative for ectromelia virus (mousepox). |
| Age | weanling |
| Strain | C3H/Mai |
| Karyotype | modal number = 78; range = 64 to 157.All cells showed 2-6 acrocentric chromosomes with secondary constrictions and 1-4 biarmed chromosomes per cells. Sixty percent of the cells had one or more minute chromosomes. |
| Derivation | The cells are SV40 transformed and are positive for SV40 T-antigen. |
| Antigen Expression | H-2k |
| Tumorigenic | No |
| Effects | No, in immunosuppressed mice Yes, in semisolid medium |
| Virus Susceptibility | Vesicular stomatitis, Glasgow (Indiana) Human poliovirus 1 |
| Virus Resistance | poliovirus 1 |
| Year of Origin | 1963 |
ATCCCCL-139 TCMK-1 ATCC細(xì)胞購(gòu)買(mǎi)
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